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Histone H3(兔杭)

貨號:GB11026

品牌:Servicebio

價格:¥650

規格: 100μl

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Product Information

 

 

Description

Histone H3.3 rabbit polyclonal

Protein full name

Histone H3.3

Synonyms

H3F3A, H3.3A, H3F3, H3 histone, family 3A, H3 histone family member 3A

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Histone H3.3

Isotype IgG
Purity Affinity purification

Predicted MW.

15 kDa

Observed MW.

15-20 kDa

Uniprot ID

P84243, P84244, P84245

 


 

Applications

 

 

Applications

WB

Species

Human, Mouse, Rat

Dilution

1: 1000

Positive tissue

Hela, Jurkat, liver cancer, lung, brain

 


 

Background

 

 

 

Histone H3.3 is a protein that in humans is encoded by the H3F3A gene.Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

             


 

Images

 

 

image.png  

Western blot analysis of Histone H3.3 (GB11026) at dilution of 1:1000.

Lane 1: Hela cell lysate

Lane 2: Jurkat cell lysate

Lane 3: RAW264.7 cell lysate

Lane 4: Human liver cancer tissue lysate

Lane 5: Mouse lung tissue lysate

Lane 6: Mouse brain tissue lysate

Lane 7: Rat lung tissue lysate

Lane 8: Rat brain tissue lysate

 


 

Storage

 

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. 

 


 

NOTE: This product is intended for research only.

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